Frozen/Freezing Cells

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Thu Jun 21 13:10:31 EST 2007


On Jun 21, 9:34 am, "Yvonne Couch" <yvonne.co... from dpag.ox.ac.uk> wrote:
> I froze some cells yesterday in one of those little portable freezing things
> in cell freezing medium and to keep it cool while clearing the hood out I
> put it in the -20.  I then COMPLETELY forgot to take the cells through to
> the -80 and was told about it today.  The person who told me said the cells
> will almost definitely be dead.  Any different opinions or should I just
> chuck them because there were about 9 vials and I don't want to waste them
> if I don't have to!
>
> Cheers
>
> Yvonne

Chuck them....



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