Frozen/Freezing Cells

[Jayakumar, R]

Thaw one of them out and check out its viability.  If they are adherent
cells, they should adhere within a couple of hours.  
Jay
 

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Yvonne Couch
Sent: Thursday, June 21, 2007 9:34 AM
To: methods from magpie.bio.indiana.edu
Subject: Frozen/Freezing Cells

I froze some cells yesterday in one of those little portable freezing
things in cell freezing medium and to keep it cool while clearing the
hood out I put it in the -20.  I then COMPLETELY forgot to take the
cells through to the -80 and was told about it today.  The person who
told me said the cells will almost definitely be dead.  Any different
opinions or should I just chuck them because there were about 9 vials
and I don't want to waste them if I don't have to!

Cheers

Yvonne

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