Ligation

Jose de las Heras via methods%40net.bio.net (by josenet from tiscali.co.uk)
Sat Jun 23 05:57:20 EST 2007


"peter" <peter.ianakiev from gmail.com> wrote in message 
news:1182476042.393099.280270 from o11g2000prd.googlegroups.com...
>
> After you digest DNA in corresponding RE buffer, add 1/10 U CIP for
> 15min at RT, PURIFY FROM GEL!!!!
> Use Qiagen gel extraction or Bio 101 kit. This will NOT interfere with
> Ligation (only Gel extractions that breakdown the agar will do so
> because of oligosaccharides)

or even just cut the band, freeze it quickly, and spin it hard in a 
microfuge for a couple of minutes. Use the liquid at the top. Not a lot of 
DNA in there, but enough for the ligation. Simple and no need for kits. It 
works for me!

Jose 




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