Circularization of small DNA fragments
(by hzhen from freeuk.com)
Thu Jun 28 22:14:27 EST 2007
> On Jun 27, 11:56 pm, "Carly Smith" <carly.sm... from anu.edu.au> wrote:
>> Hi everyone,
>> Does anyone know what the size limitations of circularizing DNA are? Is it
>> possible to circularize a fragment of 140bp?
> Should not be a problem, I have made ssDNA circles of 50 bases in the
> past using bridge oligo.
dsDNA is stiffer than ssDNA, so I'm not sure how relevant that is. But
it seems possible to circularise fragment smaller than 140bp, something
I came across (look in the paper for the further reference) -
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