Circularization of small DNA fragments

ChenHA via (by hzhen from
Thu Jun 28 22:14:27 EST 2007

peter wrote:
> On Jun 27, 11:56 pm, "Carly Smith" < from> wrote:
>> Hi everyone,
>> Does anyone know what the size limitations of circularizing DNA are? Is it
>> possible to circularize a fragment of 140bp?
>> Thanks,
>> Carly
> Should not be a problem, I have made ssDNA circles of 50 bases in the
> past using bridge oligo.
> Peter

dsDNA is stiffer than ssDNA, so I'm not sure how relevant that is.  But 
it seems possible to circularise fragment smaller than 140bp, something 
I came across (look in the paper for the further reference) -

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