Circularization of small DNA fragments

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Thu Jun 28 15:12:10 EST 2007


On Jun 27, 11:56 pm, "Carly Smith" <carly.sm... from anu.edu.au> wrote:
> Hi everyone,
>
> Does anyone know what the size limitations of circularizing DNA are? Is it
> possible to circularize a fragment of 140bp?
>
> Thanks,
> Carly

Should not be a problem, I have made ssDNA circles of 50 bases in the
past using bridge oligo.
Peter



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