Circularization of small DNA fragments

peter via (by peter.ianakiev from
Thu Jun 28 15:12:10 EST 2007

On Jun 27, 11:56 pm, "Carly Smith" < from> wrote:
> Hi everyone,
> Does anyone know what the size limitations of circularizing DNA are? Is it
> possible to circularize a fragment of 140bp?
> Thanks,
> Carly

Should not be a problem, I have made ssDNA circles of 50 bases in the
past using bridge oligo.

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