lysis buffer preserving protein-RNA interactions

Lechu via methods%40net.bio.net (by lech_kaczmarczyk from yahoo.com)
Fri Mar 2 07:10:56 EST 2007


Hi all,

Could anyone recommend me some lysis buffer for brain tissue,
preserving RNA-PROTEIN interactions. I plan to do mRNA 'pull-downs'
with rec HisTagged RNA binding protein. Want to use both, isolated
mRNA and crude cell lysate.
HEPES-based ELB buffer will do? Any other suggestions?

Thanks in advance,

Lech



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