IPTG induction in non E.coli strains

[Francesca]

Hi... 
I have a question, let me explain it: 
vector pUC18 can be used as expression vector, as it has pLac and lacZ polylinker to clone gene of interest. 
It is always suggested to clone in cells like E.coli JM109, XL1Blue etc. in order to perform the blue/white screening... ok? 
But... 
only for that? I mean. I knew that that strains were required for alfa-peptide formation and so IPTG/Xgal screening. 
Are they also necessary to study IPTG induction? 
IPTG binds to lac repressor coded from lacI, but pUC18 doesn't carry any lacI gene, so I suppose that lacI is coded from the host strains. Does it? 
I've a plasmid, based on pUC18, and trying expression in a strain of Pseudomonas. IPTG induction doesn't work. Could it be because it lacks of lacI genes in its chromosome? 

Thank you

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