DNA in buffer
(by someone from somewhere.com)
Sat Mar 3 07:27:38 EST 2007
"Jose de las Heras" <josenet from tiscali.co.uk> wrote in message
news:54pftuF21t0cmU1 from mid.individual.net...
> "Blunt Tool" <someone from somewhere.com> wrote in message
> news:t5dFh.4699$8U4.3107 from news-server.bigpond.net.au...
>> How long can you store plasmid DNA (~6kb) in TE buffer at -20C without no
> You may get some degradation, perhaps, if there's some contaminant
> nuclease... but I don't think you'll get mutations.
> It's better to store plasmids frozen in TE than as a bacterial stock in
> glycerol, where you *may* get mutations.
would you like to explain why this is so?
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