DNA in buffer

Blunt Tool via methods%40net.bio.net (by someone from somewhere.com)
Sat Mar 3 07:27:38 EST 2007


"Jose de las Heras" <josenet from tiscali.co.uk> wrote in message 
news:54pftuF21t0cmU1 from mid.individual.net...
>
> "Blunt Tool" <someone from somewhere.com> wrote in message 
> news:t5dFh.4699$8U4.3107 from news-server.bigpond.net.au...
>> Hi
>>
>> How long can you store plasmid DNA (~6kb) in TE buffer at -20C without no 
>> mutations?
>>
>> ST
>
> Mutations?
>
> You may get some degradation, perhaps, if there's some contaminant 
> nuclease... but I don't think you'll get mutations.
>
> It's better to store plasmids frozen in TE than as a bacterial stock in 
> glycerol, where you *may* get mutations.

would you like to explain why this is so?

ST 




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