method for cleaning plant DNA
Ranadheer Kumar Gupta
(by ranadheergupta from gmail.com)
Sun Mar 4 00:59:47 EST 2007
probably these protocols work for you. we got good quality DNA from Leucaena
leucocephala using one of the protocol with which we could do RAPD, Southern
C/o Prof.P.B.Kavi Kishor
Dept. of Genetics
On 3/2/07, Olga Kopp <KOPPOL from uvsc.edu> wrote:
> Does anybody know about an efficient method to clean and/or isolate DNA
> from plants that is pure enough to use for AFLP analysis. We have 2
> species of plants (Astragalus) and we isolated DNA from both. One
> species' DNA worked for the AFLP, the other one didn't. I used the CTAB
> method by Soltis (precipitation has been done with isopropanol). I have
> also used Qiagen (plant) and that didn't work neither.
> I suspect there is too much junk (polysaccharides perhaps) in the DNA
> that it is not cutting and amplifying in the AFLP.
> We only have a little amount of plant material. The plant material was
> harvested in the field and put immediately inside Drierite (this method
> works for all our other experiments).
> I will really appreciate any input.
> Olga Ruiz Kopp, Ph.D.
> Assistant Professor
> Biology Department
> Utah Valley State College
> 800 West University Parkway MS 179
> Orem, UT 84058-5999
> Tel. (801) 863-6259
> Fax: (801) 863-8064
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> Methods from net.bio.net
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