cycloheximide conc

lam via methods%40net.bio.net (by pilamca from yahoo.ca)
Sun Mar 4 20:41:02 EST 2007


Dear all,
I have been trying to use cycloheximide to inhibit
protein synthesis in stably transfected Hek293 cells. 
>From literature, people use 2-20ug/ml cycloheximide. 
I tried 0.2-20ug/ml and my cells died after 8 hrs
incubation in 1-20ug/ml.  At lower conc, there is no
inhibition.  
Does anyone know why and if I can try other
inhibitors?

Thanks.

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