[western blotting] SynGene GeneGnome system vs G-BOX
(by amanda.beardsley from gmail.com)
Mon Mar 5 17:32:50 EST 2007
This isn't about chemilumminescence, but I just wanted to add that I have
previously used the Odyssey machine for Westerns and had great success. Yes
you do have to purchase the IR dyes, but there is no ECL reagents to deal
with, and the IR dyes are quite stable. I've rescanned Westerns a few weeks
later and still got the same results (although the company say that if
stored properly they are stable for over a year- I didn't get to try that).
I like the fact that I didn't have to view the blot right away before the
signal dies down (like with chemiluminescence), and the laser intensity can
be adjusted to suit the antibody. In dealings with tech support, I also
found them to be helpful.
On 3/6/07, Pow Joshi <pow.joshi from gmail.com> wrote:
> On 4 Mar 2007 16:16:21 -0800, Lechu <lech_kaczmarczyk from yahoo.com> wrote:
> > Hello,
> > Could anyone recommend a system for chemiluminescence documentation?
> > Syngene GeneGnome /or G-BOX system? Or maybe some other? The priority
> > is reliable, reproducible Western Blots readouts (chemi). Does any
> > system dedicated for both, chemi and fluor (like Syngene's G-BOX)
> > offers sensitivity comparable to only-chemi systems (like GeneGnome)?
> > Both GeneGnome (dedicated only for chemi) and G-BOX (chemi + fluor)
> > have similar pricing and parameters (16 bit camera, cooling, 1.4
> > MegaPix, etc.), so I would go for the more versatile one (G-BOX). Can
> > the users of these system(s) comment on that?
> > Thanks in advance.
> > best, Lech
> I once had an opportunity to look at "licor Oddyssy" machine. I think
> it does dot slots rather easily, and supposed to do westerns as well
> as "immunohistochemistry stainings" ...... the only issue is that it
> uses infrared lasers to detect the signals, and ergo, one requires to
> have their specified IR dye secondaries....ergo, it is very different
> from the chemi/fluo readouts......
> you could have more info from their website I guess, if you'd be
> interested: http://www.licor.com/bio/Odyssey2/OdysseyTour_1.jsp
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