PCR from blood clot

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Wed Mar 7 23:17:10 EST 2007


On 3/7/07, Manuel Fernando Ariza Botero <mfarizab from unal.edu.co> wrote:
> Has anybody done PCR from a mammal blood clot. Is there a chance to recover
> white cells as DNA source from that mess?
> answers most welcome!!

just a shot in the dark, but could you use a combination of strong
chaotropic agents/detergents +proteases ....... and then extract the
DNA from the mess.... the caveat is that you will extract all DNA
there is, not just WBC ....

... another thought was if one could use "blood thinning agents" ...(
no not anti coagulants, but ones that dissolve the clots like say
warfarin?) ....

am being purely speculative ...and hope it triggers some ideas

best
pow
>
>
> Fernando
>
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