PCR from blood clot

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Wed Mar 7 23:17:38 EST 2007


On 3/7/07, Pow Joshi <pow.joshi from gmail.com> wrote:
> On 3/7/07, Manuel Fernando Ariza Botero <mfarizab from unal.edu.co> wrote:
> > Has anybody done PCR from a mammal blood clot. Is there a chance to
> recover
> > white cells as DNA source from that mess?
> > answers most welcome!!
>
> just a shot in the dark, but could you use a combination of strong
> chaotropic agents/detergents +proteases ....... and then extract the
> DNA from the mess.... the caveat is that you will extract all DNA
> there is, not just WBC ....
>
> ... another thought was if one could use "blood thinning agents" ...(
> no not anti coagulants, but ones that dissolve the clots like say
> warfarin?) ....
>
> am being purely speculative ...and hope it triggers some ideas
>
> best
> pow
> >
> >
> > Fernando
> >
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>



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