The top band is disappered!

Hannah Song via methods%40net.bio.net (by purifierh from gmail.com)
Sat Mar 17 16:38:58 EST 2007


Hi,

Sometimes I have a problem with that my top band of the ladder from the
Western gel is disapearing during the running. It seems like that bigger
size proteins are getting lower as they run.
However, I cannot figure out what makes this problem. It does not happen all
the time. I just noticed that it usually happens on weekends. I use the same
buffer and everything. Please help me..

thank you.


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