IPTG induction in non E.coli strains

[Aawara Chowdhury]

In <YC5Mh.14203$8U4.8322 from news-server.bigpond.net.au>,
 newsnet customer <customer from newsnet.com> wrote:

>>>> Since when did the lac operator stop being in the lac operon?  And
>>>> certainly
>>>> describing lacI to bind the lac operon is considerably more accurate 
>>>> than
>>>> your original description, "IPTG binds to the lac z operon .....".
>>>
>>> since it was first described.
>>
>> No.  Jacob and Monod described the operator as being part of the operon.
>> So you're wrong.  But that isn't surprising, given your recent posting
>> history.
>
> No. The lac operator and the lac operon are two different entities. And if 
> your saying Jacob and Monod described it differently then you need to 
> provide a reference to support that statement otherwise its rubbish... just 
> like all your postings.

ST - are you really this ignorant?

The operator is in the promoter that drives the structural genes of
the lack operon.  The operon consists of the promoter, the operator,
and the three structural genes.  Any textbook in molecular biology will 
tell you that, but as an example read Lewin's Genes. 

As for the original Jacob and Monod reference that clearly states the
operator is part of the operon, here you go:

Genetic regulatory mechanisms in the synthesis of proteins, F. Jacob, 
and J. Monod. J. Mol. Biol. 3:318-356 (1961).

If you don't have access to the original JMB article, the concept is 
clearly described in Francois Jacob's Nobel lecture from 1965 (when
he shared the prize with Lwoff, and Monod).  Jacob's Nobel lecture is
available online - here's the link:
http://nobelprize.org/nobel_prizes/medicine/laureates/1965/jacob-lecture.pdf

Monod's Nobel lecture is much shorter, and focuses on the allosteric
nature of the repressor, but also describes the operator as being at 
one end of the operon.
http://nobelprize.org/nobel_prizes/medicine/laureates/1965/monod-lecture.pdf

Finally, while there's a lot of crap on Wikipedia, they do have a reasonably
good description of the lac operon, which begins, "The lac operon is an 
operon required for the transport and metabolism of lactose in Escherichia 
coli and some other enteric bacteria. It consists of three adjacent structural
genes, a promoter, a terminator, and an operator".  The source they cite
is the original Jabob and Monod JMB paper.
http://en.wikipedia.org/wiki/Lac_operon

AC
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