Intrinsic mutation frequency in synthetic oligoes

Aawara Chowdhury via methods%40net.bio.net (by aawara from FEMA-trailer.org)
Thu Mar 22 07:01:28 EST 2007


In <1173714423.081596.43410 from c51g2000cwc.googlegroups.com>,
 peter <peter.ianakiev from gmail.com> wrote:

> Hi netters,
> I need a method to assess the mutation frequency (purity) of
> ologonucleotides - 50 -60 bp long. Any Idea how to do this?

Oligonucleotide purity is typically assessed by MALDI-TOF mass 
spec, or electrospray ionization mass spec (ESI).  There is a
relatively recent paper, titled "High-throughput analysis of
oligonucleotides using automated electrospray ionization mass 
spectrometry", by M.E. Hail et al. that compares the two 
techniques (Am. Biotech. Lab 22: 12-24).  

ESI was reliable for oligonucleotides upto 120 nt in length.  
MALDI-TOF was reliable up to about 50 nt.

AC
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