[Western Blotting] Diffusion of Antibodies in PVDF and NZ
(by legatek from hotmail.com)
Thu Mar 22 14:46:40 EST 2007
newsnet customer wrote:
>> newsnet customer <customer from newsnet.com> wrote:
>>> I have experienced protein diffusion from my membrane after an overnight
>>> antibody incubation. This was confirmed with a positive control. And
>>> he had no signal or reduced signal on his membrane, this could be a
>>> explanation. Please realise that comments on a newsgroup should be taken
>>> with a grain of salt. And you should take a chill pill.
>> So, as Kyle suggested, you are a troll. If your technique in the lab is
>> as thorough as your knowledge, there are better explanations for your
>> failed Western.
> Can you read troll? As confirmed my western failed because of the long
> incubation time. However, i'm curious to hear some of your "better
> explanations" especially when you don't know the details of my experiment or
> the experiment of the original post. Tool.
I'm willing to bet you tried to blot your protein to nylon.
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