[Western Blotting] Diffusion of Antibodies in PVDF and NZ membranes

[Kyle Legate]

newsnet customer wrote:
>> newsnet customer <customer from newsnet.com> wrote:
>>
>>> I have experienced protein diffusion from my membrane after an overnight
>>> antibody incubation. This was confirmed with a positive control. And 
>>> since
>>> he had no signal or reduced signal on his membrane, this could be a 
>>> possible
>>> explanation. Please realise that comments on a newsgroup should be taken
>>> with a grain of salt. And you should take a chill pill.
>> So, as Kyle suggested, you are a troll.  If your technique in the lab is
>> as thorough as your knowledge, there are better explanations for your
>> failed Western.
> 
> Can you read troll? As confirmed my western failed because of the long 
> incubation time. However, i'm curious to hear some of your "better 
> explanations" especially when you don't know the details of my experiment or 
> the experiment of the original post. Tool.
> 
I'm willing to bet you tried to blot your protein to nylon.