Could polyacrilamid gel (big size 38x50 cm) discriminate 4-5 bp differences?

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Fri Mar 23 10:43:48 EST 2007


On Mar 23, 7:48 am, TRAN HO QUANG <quangtra... from yahoo.com> wrote:
> Does any idea about big polyacrylamid gel (38x50cm) can distinguish two allens with 4 - 5 pb differences?. Or How many minimum bp different can be distinguished by PA gel
>
> Thanks in advance
>
> Tran Ho Quang
> Forest Science Institute of Vietnam
>
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In the old days we sequenced DNA and ran 6M urea PAGE,  so to answer
your question - gel resolution is 1bp (or less).
Peter



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