methylated DNA

AMANDA BELTRAMINI via methods%40net.bio.net (by beltramini.1 from osu.edu)
Mon Mar 26 10:01:34 EST 2007


Hi all,

Can you use methylated DNA as a PCR template? I would like to check for the presence of an insert in a plasmid isolated from Staphylococcus, and I know restriction digests aren't an option, but hoping PCR will be without further purification. Thoughts?

Thanks.



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