A Software tool to overlay two separately acquired images?

Kyle Legate via methods%40net.bio.net (by legatek from hotmail.com)
Tue Mar 27 17:29:41 EST 2007


AK wrote:
>> You can use Photoshop.  Open the files with your scans.  Copy and paste
>> Image1 into the file with Image2. You should have 2 "layers" now. Adjust 
>> opacity of the upper layer so you can see thru to the lower layer.  Then 
>> select the upper layer ("ctrl-A" for PC's), and go Edit -> Transform -> 
>> Rotate.  There will be handles on the corners and you can rotate it 
>> however much you want.
>> HTH
>> Allison
> 
> good suggestion for alignment, but what happens when he puts the opacity 
> back to 100% of the first layer?? the bottom layer will be obscured. (unless 
> layers will be blended, which requires a perfect background)
> regards
> AK
> 
> 
I don't have Photoshop in front of me so I can't remember the name of 
the window that shows the thumbnails of all layers, but in that window 
there will be the option to "screen" the layers. This will blend 
everything to be equally visible. I use it often to create colourized 
immunofluorescent overlays from monochrome pictures. I think it would 
suit Wo's purposes nicely.


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