(no subject)

[peter]

On Mar 28, 1:36 pm, "Orsini  Elena" <e... from ethz.ch> wrote:
> Hi,
>
> I have a problem in my sequencing results. I cloned a GUSNOS insert into
> a plasmid and afterwards a promoter in front. When I received my
> sequencing results the forward sequence of my promoter was fine but the
> reverse ( the annealing was at the beginning of the GUS gene) was
> superimposed after 130 base pares. This happened exactly at the level of
> the cloning site before the GUS fusion.....
>
> I suspect that my culture contains a mixture of two plasmids, the
> transformed and the not transformed....or in the worst case two
> different repetitive sequence of the GUSNOS.
>
> How could I figure this out?
>
> Is it possible to separate eventually the two plasmid?
>
> Thank you very much
>
> elena

Yep, By re-plating and picking up a single colony. Or if you wish by
re transforming your plasmid (mix of two plasmids).
Peter