Could polyacrilamid gel (big size 38x50 cm) discriminate 4-5 bp differences?

Dr. Hiranya S. Roychowdhury via methods%40net.bio.net (by hroychow from nmsu.edu)
Fri Mar 30 10:50:51 EST 2007


Regular (O---l---d) method of sequencing - with UREA-PAGE - easily 
distinguishes 1bp!  Mini versions of such gels were also available at one time.

Quoting TRAN HO QUANG <quangtranho from yahoo.com>:

> Does any idea about big polyacrylamid gel (38x50cm) can distinguish two
> allens with 4 - 5 pb differences?. Or How many minimum bp different can
> be distinguished by PA gel   
> 
> Thanks in advance
> 
> Tran Ho Quang
> Forest Science Institute of Vietnam
>  
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-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Dept. of Health and Public Services, Rm# 191T,
Dona Ana Community College of New Mexico State University
MSC 3DA,
Las Cruces, NM 88003



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