various protein A resins - difference in labolatory practice

[Dr Engelbert Buxbaum]

Am 30.04.2007, 07:58 Uhr, schrieb Lechu <lech_kaczmarczyk from yahoo.com>:

> Hi all,
>
> Is there any difference between the protein A agarose (CNBr coupled)
> protein A agarose (aldehyde bond-coupled) and protein A sepharose 4B.
> What is the difference, if any?

The coupling reaction. And as a result the stability of the protein-gel  
bond, especially after  multiple uses.

Btw: For applications like immunoprecipitation you don't need the gel  
coupled to the purified protein A. Instead, use killed bacteria expressing  
the protein on their surface. Calbiochem for example floggs that as  
"Pansorbin". Much cheaper and works just as well.