various protein A resins - difference in labolatory practice

[Nick Theodorakis]

On May 3, 10:27 am, "Dr Engelbert Buxbaum"
<engelbert_buxb... from hotmail.com> wrote:
> Am 30.04.2007, 07:58 Uhr, schrieb Lechu <lech_kaczmarc... from yahoo.com>:
>
> > Hi all,
>
> > Is there any difference between the protein A agarose (CNBr coupled)
> > protein A agarose (aldehyde bond-coupled) and protein A sepharose 4B.
> > What is the difference, if any?
>
> The coupling reaction. And as a result the stability of the protein-gel  
> bond, especially after  multiple uses.
>
> Btw: For applications like immunoprecipitation you don't need the gel  
> coupled to the purified protein A. Instead, use killed bacteria expressing  
> the protein on their surface. Calbiochem for example floggs that as  
> "Pansorbin". Much cheaper and works just as well.

It can be dirtier, as well, but some of that can be overcome by pre-
absrobing the lysate with Pansorbin first.

Also, (regarding the OP) I believe that Sepharose is a trade name for
a form of agarose.

Nick

--
Nick Theodorakis
nick_theodorakis from hotmail.com
contact form:
http://theodorakis.net/contact.html