Problem with Polyacrylamide gel

Yulia Shifrin via methods%40net.bio.net (by yulia24 from gmail.com)
Thu May 10 09:30:22 EST 2007


I would suggest preparing fresh 10%APS and washing the plates more
thoroughly to remove any residual "dirt". Also, check your TEMED

On 5/10/07, Siddhartha Parida <sidharth4 from gmail.com> wrote:
>
> I have been using  15cm X 16 cm plates for setting polyacrylamide gels
> (29:1) 10% stacking (20ml volume), but after pouring when the
> polymerization sets-in I find wave patterns in the gel
>
> any remedies ?
>
> --
> Sidharth Parida
> Senior Faculty
> Center for biotechnology and Research
> Neelachal Inst of Medical Sciences
> O.C.H.C Complex, Block - III (Near Ram Mandir)
> Janpath,Unit-3,Bhubaneswar- 751001
> ORISSA
> 09437089337(M)
>
> sidharth4 from gmail.com
> www.nimsindia.com
>
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