Problem with Polyacrylamide gel
(by ben.long from yourfinger.anu.edu.au)
Sun May 13 23:51:09 EST 2007
> In article <1178873864.366594.161010 from w5g2000hsg.googlegroups.com>, StewJW <stewjw from gmail.com> wrote:
>> Use Invitrogen Midi or smaller mini precast gels and save a lot of
> This is a non-solution. What do you think would happen if for every trivial
> problem (like polymerizing AA gel) you suggest buying a product that
> bypasses the problem altogether? What's next? - Hire a writer to write
> a grant for me?
My 2 cents...
Pre-cast gels, like almost every product you can get from Life Sciences
companies, are an example of scientific progress that makes life easier
and avoids unneeded time wasting ans stuff-ups. Why bother pouring your
own when in the same time it takes you to clean the plates, make up the
solutions, pour the gel, let it set, pour the stacker... you can have a
pre-cast gel run and stained with results in your lab-book. I have no
problem with someone who wants to do it the 'traditional' way (at least
they understand the science behind it) but where's the harm in getting
results quicker. Do you propose we synthesise our own ATP and draw our
own glass pipettes before running enzyme assays?? Yes, people had to do
that once too!
My personal recommendation is get the pre-cast gels and save yourself
time and effort! I only suggest that you make an effort to know what
you're doing!! :-)
BTW, buying a product that bypasses the problem altogether DK!?!?!
Presumably the problem is the scientific question, not whether or not a
gel is setting properly? I'd bypass a problem altogether any day!
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