mouse embryonic fibroblasts: why sodium pyruvate in medium?

AK via methods%40net.bio.net (by akhan357 from sbcglobal.net)
Thu May 17 16:53:13 EST 2007


http://www.protocol-online.org/biology-forums/posts/7031.html

Na-pyruvate is an additional energy source for the cells. They should be 
okay without it, but if your cells are growing rapidly (e.g. some cancer 
cell lines) you should consider adding it.

-Pallas-

--------------------------------------------------------------------------------

Hi,

According to the ATCC, sodium pyruvate also protects your cells from 
fluorescent lights induced damage.

-Cal-

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Pyruvate also helps to reduce the potential for cell membrane damage; a 
protective agent. Will bind and help stabilize hydrogen peroxide, protect 
against oxidative stress by binding hydrogen peroxide.

-angelak-


"Antonio" <Antonio.Sarikas from gmail.com> wrote in message 
news:1179435519.575291.78430 from h2g2000hsg.googlegroups.com...
> Why do you add sodium pyruvate to the DMEM?
>
> Thanks
> 




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