mouse embryonic fibroblasts: why sodium pyruvate in medium?
(by jongh711 from planet.nl)
Thu May 17 18:25:51 EST 2007
"Antonio" <Antonio.Sarikas from gmail.com> wrote in message
news:1179435519.575291.78430 from h2g2000hsg.googlegroups.com...
> Why do you add sodium pyruvate to the DMEM?
pyruvate is needed as a source of energy.
The man who invented the medium eventually came up with the substances that
were (minimally) needed for growth of mouse fibroblasts ; pyruvate was just
one of them (he guessed)
One of the difficulties of cell culture is the composition of the medium :
substances don't dissolve , are unstable etc , etc
Here are a few choises
1) Buy or make the complete medium and store it in small aliquotes in -20
degrees ; thaw right before use ; don't use more than a few freeze - thaw
cycles (my choise)
2)Buy the DMEM complete solution , store it at 6 degrees and use it within a
3)Buy the DMEM solution without glucose and pyruvate , store it at 6
degrees and add glucose and pyruvate from a stock solution right before you
use it. (These two are the main unstable ones in solution)
4)Buy the complete powder and now add the sodium bicarbonate after
dissolving it because in a solid the bicarbonate is the problem but glucose
and pyruvate are now stable.
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