Southern Blot

[Anne]

By PCR? You cut the nylonmembrane and run a PCR with it or how do you do it?

"peter" <peter.ianakiev from gmail.com> schrieb im Newsbeitrag 
news:1179930831.833752.233410 from g4g2000hsf.googlegroups.com...
> On May 23, 8:29 am, "Anne" <Anne.Roess... from klinik.uni-regensburg.de>
> wrote:
>> Hello,
>> does anybody know if it is possible to get the DNA out of the 
>> nylonmembrane
>> after crosslinking ? I have to know the sequences next to the binding 
>> site
>> of my sensor.
>>
>> Thanks a lot
>> Anne
>
> Yep, by PCR....
>