Good Sites in pUC-type vectors for inserting fragments?

[Aawara Chowdhury]

In <1180110858.134362.178180 from p47g2000hsd.googlegroups.com>,
 VioletBlueCrayon from gmail.com <VioletBlueCrayon from gmail.com> wrote:

> Hello,
> I would like to make a derivative of a pUC-type vector, namely the
> addition of another antibiotic resistance cassette. I would like to do
> this away from the polylinker region.
>
> Is there a good restriction site in the backbone of the vector for
> this?

I have inserted fragments into the AatII recognition site in pUC19.  This 
site is between the alpha fragment of lacZ and the beta-lactamase gene.

AC
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