Southern Blot

Anne via methods%40net.bio.net (by Anne.Roessger from klinik.uni-regensburg.de)
Sun May 27 07:16:48 EST 2007


I already tried inverse PCR like each other method (RACE, RS-PCR) and it 
didn`t work. But I´ll try again.
Thanks for the link to  PCRLinks.com.

"Aawara Chowdhury" <aawara from FEMA-trailer.org> schrieb im Newsbeitrag 
news:cUz5i.16306$qJ1.15037 from newsfe18.lga...
> In <drt5i.126$wR3.40 from newsfe03.lga>,
> DK <dk from no.email.thankstospam.net> wrote:
>
>> In article <5blqrsF2tkoopU1 from mid.dfncis.de>, "Anne" 
>> <Anne.Roessger from klinik.uni-regensburg.de> wrote:
>>>
>>>Thank you Peter for your help.
>>>I´ll try to find the sequences next to my sensor then by RACE or
>>>restriction-site PCR. I try to find the integration site of my used
>>>transposon. If anybody does know another method to find the integration
>>>sites it would be very helpful for me.
>>
>> Why not just prime off your transposon and sequence?
>> What am I missing?
>
> Or, if you really want to clone the transposon insertion site(s), make use 
> of
> inverse PCR, which is typically how transposon (or retrovirus) insertion 
> sites
> are cloned ...
> <http://www.pcrlinks.com/variants/inverse_pcr.htm>
>
> AC
> -- 
> Email: echo 36434455860060025978157675027927670979097959886449930P | dc 




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