HUH7 cells maintainance

helena de souza pereira via (by helena from
Thu May 31 09:52:59 EST 2007

I have a very big problem with maintaining Huh7 human hepatocarcinoma
cell line, I use DMEN (without sodium pyruvate) as medium with 1X Pen-Str 
and 10% Foetal Calf (Hyclone)serum... I trypsinize them and afterwards they 
are in apoptosis process.


helena de Souza Pereira

Universidade Federal do Rio de Janeiro
Instituto de Biologia
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