Description about the pUC vectors

[KiranBabu Tiwari]

Hi,

I am working about the cloning and expression of cry proteins from Bt
(isolated from Khumbu, Mt. Everest region, Nepal) DNNA in E. coli HB 101. I
should know about the cloning capacity and expression capacity of the
vectors. Ya, I clonned 4.6kb fragment (of the Bt DNA library) with cry3
specific fragment amplification using universal primers. I didn't get
success to express yet. So, please give me more informations about the
compatibility of the vectors and the host in order to express the cry genes.
The help will be acknowledged.

Thanking you.............

Kiran Babu Tiwari
gp120cdnashuffling from gmail.com