Antibody binding to nonpermeabilized cells

Beug, Shawn via (by sbeug from
Tue Nov 6 14:06:58 EST 2007

Hi all,


I am interested in calculating the ratio of intracellular and
extracellular GFP-tagged protein in primary neurons. I am trying to
demonstrate that perturbation of posttranslational modification affects
extracellular/intracellular sublocalization. I was wondering if
immunnostaining for transfected neurons with an anti-GFP antibody (using
a Cy3-conjugated secondary antibody) would be the best way to calculate
this ratio. I have done this before in the past but am unsure if
antibodies would stain intracellular proteins, and as a result, give me
the wrong numbers. 


Cheers, Shawn


Confidentiality Statement - The contents of this e-mail, including its attachment, are intended for the exclusive use of the recipient and may contain confidential or privileged information.  If you are not the intended recipient, you are strictly prohibited from reading, using, disclosing, copying, or distributing this e-mail or any of its contents.  If you received this e-mail in error, please notify the sender by reply e-mail immediately or the Privacy Office (privacy from ) and permanently delete this e-mail and its attachments, along with any copies thereof.  Thank you.

Avis de confidentialité – Ce courriel, y compris ses pièces jointes, s’adresse au destinataire uniquement et pourrait contenir des renseignements confidentiels. Si vous n’êtes pas le bon destinataire, il est strictement interdit de lire, d’utiliser, de divulguer, de copier ou de diffuser ce courriel ou son contenu, en partie ou en entier. Si vous avez reçu ce courriel par erreur, veuillez en informer immédiatement l’expéditeur ou le bureau de la Protection des renseignements personnels (info.privee from, puis effacez le courriel ainsi que les pièces jointes et toute autre copie. Merci.

More information about the Methods mailing list