Acid Phenol/chloroform

Ed Siefker via methods%40net.bio.net (by ebs15242 from creighton.edu)
Tue Nov 6 15:10:43 EST 2007


I got a kit from Ambion to do some RACE, this kit requires acid 
phenol/chloroform but doesn't tell me what the composition of this is. 
I called Ambion and they didn't know.  I couldn't find a recipe in 
Maniatis either.

I have a brand new bottle of phenol ph 6.7. What do I need to add to 
make "acid phenol/chloroform"?  Thanks.
-Ed



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