Acid Phenol/chloroform

[Sudheendra Rao N R]

Hi,
I got this from ambion website :) i hope this will help you.
http://www.ambion.com/techlib/tb/tb_158.html

On Nov 7, 2007 1:40 AM, Ed Siefker <ebs15242 from creighton.edu> wrote:

> I got a kit from Ambion to do some RACE, this kit requires acid
> phenol/chloroform but doesn't tell me what the composition of this is.
> I called Ambion and they didn't know.  I couldn't find a recipe in
> Maniatis either.
>
> I have a brand new bottle of phenol ph 6.7. What do I need to add to
> make "acid phenol/chloroform"?  Thanks.
> -Ed
>
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