Acid Phenol/chloroform

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Wed Nov 7 10:48:50 EST 2007


On 06/11/2007, Ed Siefker <ebs15242 from creighton.edu> wrote:
>
> I got a kit from Ambion to do some RACE, this kit requires acid
> phenol/chloroform but doesn't tell me what the composition of this is.
> I called Ambion and they didn't know.  I couldn't find a recipe in
> Maniatis either.
>
> I have a brand new bottle of phenol ph 6.7. What do I need to add to
> make "acid phenol/chloroform"?  Thank




You can get the recipie for making the same from either Sambrook etal., or
> Current methods in molecular biology.



Best
Pow

-Ed
>
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