Acid Phenol/chloroform

Jose de las Heras via (by josenet from
Thu Nov 8 20:26:23 EST 2007

"Dr. Hiranya S. Roychowdhury" <hroychow from> wrote in message 
news:mailman.535.1194542935.23109.methods from
> The pH of the acid phenol is usually kept around 4.0.
> We used to make it in lab with Tri.HCl, but Sigma sells the stuff for so
> cheap that it doesn't pay to even equilibrate your own.  Sigma sells both
> Phenol and Phenol Chloroform with pH8.0 or pH4 buffers.

same here, unless you need industrial amounts, the (little) extra expense is 
worth it to avoid hassle/handling.


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