96-well plate DNA extraction kits
(by C.Thompson from MARLAB.AC.UK)
Sun Nov 11 08:48:20 EST 2007
I am usually loathe to resort to commercial kit methods for DNA
extraction, but working with moulluscs and local
prohibitions on phenol/chloroform, it's maybe time to start.
So far I have been using Qiagen spin column kits very successfully, but
I need to move to a 96-well plate format.
Are there any 96-well plate extraction kits available that can be spun
in Sigma 2-16K or 3K15 centrifuges with 11123 / 11223
rotors and 13223 buckets?
Eg Invitrogen kits can indeed be spun at the relatively slow speeds on
these centrifuges (approx 2250g) - but the brochure
suggests a plate height clearance of 5.75cm, and I have no idea where
that is measured from, the ends of the bucket,
the sides of the bucket?
Conservation & Restoration Group
FRS Freshwater Laboratory
Faskally, Pitlochry, Perthshire PH16 5LB
direct 01224 294462
switch 01796 472060
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