What absorbs intensely at 260nm?

Tom Anderson via methods%40net.bio.net (by ucgatan from ucl.ac.uk)
Tue Nov 13 13:57:53 EST 2007


On Tue, 13 Nov 2007, Ed Siefker wrote:

> What could have possibly gotten in my DNA to do this?

Silica absorbs very strongly down there; if you used a silica-based
cleanup kit anywhere in the pipeline (which you didn't, as far as i can
see), that would do it.

If you have a scanning spec, try running a spectrum and having a look.
Silica is a big wall at ~260 and below.

If you don't manage to get any sense from the spec, i guess it's time for
a quantitative gel. Pain in the caudal extremity!

tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson from ucl.ac.uk



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