speI xbaI sublconing
(by vadivelarunachalam from yahoo.com)
Thu Nov 29 04:06:58 EST 2007
I am facing problems in cohesive subcloning a 500 bp insert from the double digest of SpeI and XbaI onto a vector of 13.5 Kb size at the same sites. Please suggest me is it correct strategy as these two enzymes generate similar ends though differ in recognition sites.
Never miss a thing. Make Yahoo your home page.
More information about the Methods