speI xbaI sublconing

V Arunachalam via methods%40net.bio.net (by vadivelarunachalam from yahoo.com)
Thu Nov 29 04:06:58 EST 2007


Hi, 
I am facing problems in cohesive subcloning a 500 bp insert from the double digest of SpeI and XbaI onto a vector of 13.5 Kb size at the same sites. Please suggest me is it correct strategy as these two enzymes generate similar ends though differ in recognition sites.


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