Methods Digest, Vol 30, Issue 20

doaa zineldeen via (by zineldeen from
Fri Nov 30 20:54:26 EST 2007

I am having problem in detection of my protein which is a 72 KD kinase by
western blotting
I am using a commercial one , it could detect my kinase by IF howevere on
western blotting many faint bands are seen and high background
I tried different blocking and different dilutions of the primary and
secondary and increasedwashing time but no clear band
anyone can help
thank you

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