Methods Digest, Vol 30, Issue 20

doaa zineldeen via methods%40net.bio.net (by zineldeen from gmail.com)
Fri Nov 30 20:54:26 EST 2007


hello
I am having problem in detection of my protein which is a 72 KD kinase by
western blotting
I am using a commercial one , it could detect my kinase by IF howevere on
western blotting many faint bands are seen and high background
I tried different blocking and different dilutions of the primary and
secondary and increasedwashing time but no clear band
anyone can help
thank you


More information about the Methods mailing list