About DNA sequencing
kou
via methods%40net.bio.net
(by koustubhvaze2 from googlemail.com)
Sun Oct 7 12:12:42 EST 2007
Hello everybody,
I am PhD stdent and working on circadian rhythms in Drsophila.
I am planning to do sequencing of period locus for detecting
polymorphism.
I have a very basic question about sequencing :
After sequencing, entire sequence is not accurate. stretch of sequence
immediately after primers is not accurate.
So my question is
How to choose accurate sequence ?
I mean what should be the start point and end point of accurate
sequence with respect to primer position?
Kou
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