Oligos labelling

3pi via methods%40net.bio.net (by patrick.demarta from gmail.com)
Tue Oct 9 08:32:43 EST 2007


Hello all,

The task i'm facing is labelling oligonucleotides with biotin-dutp by
reverse-transcription on their mRNA targets.
Is there anyone who has some experience in it? I'm still having some
poor results, and often non-specific labelling occurs.

Thanks alot in advance.

Patrick.



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