(by novalidaddress from nurfuerspam.de)
Wed Oct 10 12:50:42 EST 2007
On Oct 10, 1:09 pm, "Sudheendra Rao N R" <sudhe... from gmail.com> wrote:
formaldehyde reacts reversibly with amino groups (eg lysines) and may
interfere with epitope recognition. When you include some amino- or
ammonium compounds (eg NH4HCO3) in excess in your sample prep, they
should scavenge the formaldehyde and it should work. Maybe even the
glycin in your running buffer is enough.
> Hello all,
> can we western blot using protein extract from the formalin fixed tissues?
> Does perfusion of tissue (say brain) has anything to do with western blot
> based protein detection?
> Thanks in advance
> National Brain Research Centre
> Think before agree
> Think before you nod
> but STOP thinking
> and You Are God
More information about the Methods