About DNA sequencing
(by ebs15242 from creighton.edu)
Thu Oct 11 13:04:13 EST 2007
With our sequencing facility, I get readable sequence after about 20-30
bases. I'd design my primers so they're around 50bp 5' of the sequence
you want just to be safe. Hope this helps
> Hello everybody,
> I am PhD stdent and working on circadian rhythms in Drsophila.
> I am planning to do sequencing of period locus for detecting
> I have a very basic question about sequencing :
> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.
> So my question is
> How to choose accurate sequence ?
> I mean what should be the start point and end point of accurate
> sequence with respect to primer position?
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