About DNA sequencing

Ed Siefker via methods%40net.bio.net (by ebs15242 from creighton.edu)
Thu Oct 11 13:04:13 EST 2007


With our sequencing facility, I get readable sequence after about 20-30 
bases.  I'd design my primers so they're around 50bp 5' of the sequence 
you want just to be safe.  Hope this helps
-Ed

kou wrote:
> Hello everybody,
> 
>  I am PhD stdent and working on circadian rhythms in Drsophila.
> 
> I am planning to do sequencing of period locus for detecting
> polymorphism.
> 
> I have a very basic question about sequencing :
> 
> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.
> So my question is
> 
> How to choose accurate sequence ?
>  I mean what should be the start  point and end point  of accurate
> sequence with respect to primer position?
> 
> Kou
> 
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods




More information about the Methods mailing list