About DNA sequencing

[Ed Siefker]

With our sequencing facility, I get readable sequence after about 20-30 
bases.  I'd design my primers so they're around 50bp 5' of the sequence 
you want just to be safe.  Hope this helps
-Ed

kou wrote:
> Hello everybody,
> 
>  I am PhD stdent and working on circadian rhythms in Drsophila.
> 
> I am planning to do sequencing of period locus for detecting
> polymorphism.
> 
> I have a very basic question about sequencing :
> 
> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.
> So my question is
> 
> How to choose accurate sequence ?
>  I mean what should be the start  point and end point  of accurate
> sequence with respect to primer position?
> 
> Kou
> 
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