About DNA sequencing

[Maximilian Haeussler]

>
> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.
>

Just in case: I had the same problem. If you're using TOPO TA cloning into
pCRII like me than the problem might be well-known and can be remedied by
changing the primers:
http://www.nd.edu/~aforbes/Forbesetal-biotech.pdf

hope it helps someone,
Max