About DNA sequencing

Maximilian Haeussler via methods%40net.bio.net (by maximilianh from gmail.com)
Fri Oct 12 04:57:12 EST 2007

> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.

Just in case: I had the same problem. If you're using TOPO TA cloning into
pCRII like me than the problem might be well-known and can be remedied by
changing the primers:

hope it helps someone,

More information about the Methods mailing list