About DNA sequencing

Maximilian Haeussler via methods%40net.bio.net (by maximilianh from gmail.com)
Fri Oct 12 04:57:12 EST 2007


>
> After sequencing, entire sequence is not accurate. stretch of sequence
> immediately after primers is not accurate.
>

Just in case: I had the same problem. If you're using TOPO TA cloning into
pCRII like me than the problem might be well-known and can be remedied by
changing the primers:
http://www.nd.edu/~aforbes/Forbesetal-biotech.pdf

hope it helps someone,
Max


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