protein DNA crosslinked complex purification

kaj.stenberg from helsinki.fi.invalid via methods%40net.bio.net (by kaj.stenberg from helsinki.fi.invalid)
Fri Oct 19 14:08:24 EST 2007


qiang fu <nanoqfu from yahoo.com> wrote:
> Hi, Guys:
>    
>   I am recently doing some UV crosslink between the protein  and DNA.(Histone protein and DNA)
>   Due to our small amount sample about 50 ul and low percentage of successful crosslink. Is there any method to isolate the crosslinked sample out from the DNA with no protein crosslinked.
>    
>   Your suggestions will be great helpful.

Could you run an EMSA on a preparative scale?
http://www.mobitec.de/de/companies/prod_molpr_nucleic.html

-- 
Kaj


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