Stripping western blots
(by aawara from FEMA-trailer.org)
Fri Oct 26 07:36:47 EST 2007
In <mailman.365.1193341560.23109.methods from net.bio.net>,
Ann Sutherland <as9n from virginia.edu> wrote:
> What is the best way to strip Western blots to preserve protein for
> the next round of antibody?
We use this stuff that Pierce makes called "Restore".
It works very well.
> We are doing Western blots of mouse
> embryos, so we have very little protein to begin with, and have been
> having trouble re-probing. Either the stripping method isn't strong
> enough to remove the first antibody, or it removes everything.
What method are you using?
> are using nitrocellulose membrane, does PVDF work better?
Restore works with nitrocellulose and PVDF.
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