Stripping western blots

Aawara Chowdhury via methods%40net.bio.net (by aawara from FEMA-trailer.org)
Fri Oct 26 07:36:47 EST 2007


In <mailman.365.1193341560.23109.methods from net.bio.net>,
 Ann Sutherland <as9n from virginia.edu> wrote:

>
> What is the best way to strip Western blots to preserve protein for 
> the next round of antibody? 

We use this stuff that Pierce makes called "Restore".
<http://www.piercenet.com/products/browse.cfm?fldID=02040210>

It works very well.

>  We are doing Western blots of mouse 
> embryos, so we have very little protein to begin with, and have been 
> having trouble re-probing.  Either the stripping method isn't strong 
> enough to remove the first antibody, or it removes everything.  

What method are you using?

> We 
> are using nitrocellulose membrane, does PVDF work better?

Restore works with nitrocellulose and PVDF.

AC
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