why EDTA is used for lysozyme action

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Wed Oct 31 07:32:22 EST 2007


Am 30.10.2007, 14:11 Uhr, schrieb chiranjit chowdhury <cjtcdy from gmail.com>:

> I have gone through several literature where
> they have used EDTA with lysozyme mediated cell lysis of Gram negative
> bacteria but not in case of Gram positive bacteria.
> Can anybody tell me why EDTA is used specially for Gram negative  
> bacteria,
> the exact mechanism behind it

The function of EDTA is to complex metal ions, which are required as  
cofactors for some proteases and other hydrolases. Heavy metals may also  
inactivate enzymes e.g. by reacting with sulfhydryl groups.


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